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KMID : 0357119960180010001
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Korean Journal of Immunology
1996 Volume.18 No. 1 p.1 ~ p.12
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Immunopathologic Response of Human Mononuclear Cells and Mouse Peritoneum to Bacteroides fragilis -TNFa Gene Expression in Mononuclear Cells and Peritoneal Tissue Infected with B.fragilis
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김정목
한승룡/조양자/정현재/김정룡
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Abstract
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Objective: Bacteroides fragilis is one of the most frequently isolated anaerobes in tissues of intraabdominal infection, particularly in intraabdominal abscess. In experimental model with acute intraabdominal infection, the response to B.
fragilis
is
characterized by infiltration of neutrophils and monocytes. To understand the pathogenesis of B. fragilis infection, it is important to explore the mechanism for inflammatory signals such as proinflammatory cytokines induced by B. fragilis. The
goal of
this study was to determine whether monocytes infected with B. fragilis could express TNFa or not. @ES Methods:@EN After human peripheral blood mononuclear cells(PBMC) were infected with B. fragilis for 1, 4 and 9 hours, RNAs were extracted from
the
BPMC. And then TNFa mRNA expression was assessed using reverse transcriptional polymerase chain reaction (RT-PCR). The expressed TNF-a transcripts were quantified by RT-PCR using synthetic standard RNA. TNFa proteins in culture supernatants were
also
measured by enzyme-linked immunosorbent assay (ELISA). @ES Results:@EN 1) TNFa mRNA was highly expressed in 100:1 of ratio of B. fragilis : PBM(7.4×10E7 molecules/μg of total RNA). However, it was lower expressed in 10:1 than in 100:1 of the
ratio. 2)
TNFa mRNA was initially expressed in PBMC at 4 hours(5.1×10E5 molecules/μg of total RNA) and reached to peak at 9 hours(4.7×10E7 molecules/μg of total RNA). Number of molecules of the expressed TNFa transcripts was parallel to amounts of
proteins
secreted from PBMC. 3) TNFa mRNA WAS also upregulated in mouse tissues infected with B.fragilis. 4) TNFa expression by B. fragilis infection was induced through Ca++-dependent protein kinase C pathway. Conclusion: These results suggest that TNFa
induced
by B. fragilis play a role in the pathogenesis of B. fragilis infection such as intraabdominal abscess.
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